Pool-Sequencing library of Ameiurus melas phenotypic males - SRA

SRX14027782: Pool-Sequencing library of Ameiurus melas phenotypic males
1 ILLUMINA (Illumina NovaSeq 6000) run: 28.2M spots, 14.1G bases, 8.1Gb downloads

Design: Pool-sequencing libraries were prepared using the Illumina TruSeq Nano DNA HT Library Prep Kit (Illumina, San Diego, CA) according to the manufacturer's protocol. After the fragmentation of each gDNA pool (200 ng/pool) by sonication using an M220 Focused-ultrasonicator (COVARIS), the size selection was performed using SPB beads retaining fragments of 550 bp. Following the 3' ends of blunt fragments mono-adenylation and the ligation to specific paired-end adaptors, the amplification of the construction was performed using Illumina-specific primers. Library quality was verified with a Fragment Analyzer (Advanced Analytical Technologies) and then quantified by qPCR using the Kapa Library Quantification Kit (Roche Diagnostics Corp, Indianapolis, IN). The enriched male and female pool libraries were then sequenced using a paired-end multiplexed sequencing mode on a NovaSeq S4 lane (Illumina, San Diego, CA), combining the two pools on the same lane and producing 2 x 150 nt with Illumina NovaSeq Reagent Kits according to the manufacturer's instructions.

Submitted by: INRAE (Fish Physiology and Genomics laboratory)

Study: Ameiurus melas isolate:M_S1 Genome sequencing and assembly

PRJNA603265 • SRP300856 • All experiments • All runs

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Genome sequence of the black bullhead catfish (Ameiurus melas)

Sample: Ameiurus melas Pool Males

SAMN25294298 • SRS11863946 • All experiments • All runs

Organism: Ameiurus melas

Library:

Name: Ameiurus_melas_Mpool

Instrument: Illumina NovaSeq 6000

Strategy: WGS